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Analysis of Processing, Post-Maturation, and By-Products of shRNA in Gene and Cell Therapy Applications

Short hairpin RNAs (shRNAs) are potent tools for gene silencing, offering therapeutic potential for gene and cell therapy applications. However, their efficacy and safety depend on precise processing by the RNA interference machinery and the generation of minimal by-products. In this protocol, we de...

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Huvudupphov: Zhenyi Hong, Nikola Tesic, Xavier Bofill-De Ros
Materialtyp: Artigo
Språk:Inglês
Utgiven: MDPI AG 2025-04-01
Serie:Methods and Protocols
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Länkar:https://www.mdpi.com/2409-9279/8/2/38
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