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Mutational Scanning of PCR Products by Subtractive Oligonucleotide Hybridization Analysis

Here, we describe a new approach for mutational scanning of PCR products through hybridization analysis between complementary oligonucleotides. Sets of overlapping probe oligonucleotides complementary to wild-type (WT) sequence are hybridized to microbead-immobilized PCR products under solution-like...

Ausführliche Beschreibung

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Bibliografische Detailangaben
Hauptverfasser: P. Nilsson, A. Larsson, J. Lundeberg, M. Uhlén, P.-Å. Nygren
Format: Artigo
Sprache:Inglês
Veröffentlicht: Taylor & Francis Group 1999-02-01
Schriftenreihe:BioTechniques
Online-Zugang:https://www.future-science.com/doi/10.2144/99262rr01
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