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Optimized high-throughput whole-genome sequencing workflow for surveillance of influenza A virus

Abstract Whole-genome sequencing (WGS) is essential for monitoring the genetic diversity of influenza A virus (IAV) across host species. We optimized a multisegment RT-PCR (mRT-PCR) protocol to enhance amplification of all eight IAV segments using modified RT and PCR conditions. Additionally, we int...

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Principais autores: Matthias Licheri, Mike Mwanga, Manon F. Licheri, Annika Graaf-Rau, Cora Sägesser, Pascal Bittel, Timm Harder, Franziska Suter-Riniker, Jenna N. Kelly, Ronald Dijkman
Format: Artigo
Sprog:Inglês
Udgivet: BMC 2025-09-01
Serier:Genome Medicine
Fag:
Influenza A virus
Whole-genome sequencing
Oxford Nanopore sequencing
Swine IAV
Avian IAV
Human IAV
Online adgang:https://doi.org/10.1186/s13073-025-01512-x
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