Marker-free genome editing in the edible mushroom, Pleurotus ostreatus, using transient expression of genes required for CRISPR/Cas9 and for selection

Abstract In a previous study, we reported a transient transformation system using repeated screening for hygromycin B (Hyg) resistance in the basidiomycete Ceriporiopsis subvermispora. In the present study, by combining this technique with CRISPR/Cas9, we demonstrated successful marker-free genome e...

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Detalhes bibliográficos

Principais autores:	Daishiro Koshi, Hiroki Ueshima, Moriyuki Kawauchi, Takehito Nakazawa, Masahiro Sakamoto, Mana Hirata, Kosuke Izumitsu, Takuya Sumita, Toshikazu Irie, Yoichi Honda
Formato:	Artigo
Idioma:	Inglês
Publicado em:	SpringerOpen 2022-05-01
Colecção:	Journal of Wood Science
Assuntos:	Transient transformation Genome editing Agaricomycete Mushroom Wood-rot fungi Pleurotus ostreatus
Acesso em linha:	https://doi.org/10.1186/s10086-022-02033-6
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