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Expression and secretion of glycosylated barley oxalate oxidase in Pichia pastoris.

Oxalate oxidase is an enzyme that degrades oxalate and is used in commercial urinary assays to measure oxalate levels. The objective of this study was to establish an enhanced expression system for secretion and purification of oxalate oxidase using Pichia pastoris. A codon optimized synthetic oxala...

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Detalles Bibliográficos
Principais autores: William Donelan, ShiWu Li, Paul R Dominguez-Gutierrez, Augustus Anderson Iv, Li-Jun Yang, Cuong Nguyen, Benjamin K Canales
Formato: Artigo
Idioma:Inglês
Publicado: Public Library of Science (PLoS) 2023-01-01
Series:PLoS ONE
Acceso en liña:https://doi.org/10.1371/journal.pone.0285556
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