Protocol for RNA-seq library preparation starting from a rare muscle stem cell population or a limited number of mouse embryonic stem cells

Summary: It remains challenging to generate reproducible, high-quality cDNA libraries from RNA derived from rare cell populations. Here, we describe a protocol for high-throughput RNA-seq library preparation, including isolation of 200 skeletal muscle stem cells from mouse tibialis anterior muscle b...

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Detalhes bibliográficos

Principais autores:	Stefania Dell’Orso, Aster H. Juan, Victoria Moiseeva, Laura García-Prat, Pura Muñoz-Cánoves, Vittorio Sartorelli
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Elsevier 2021-06-01
coleção:	STAR Protocols
Assuntos:	Cell isolation Flow Cytometry/Mass Cytometry RNA-seq Molecular Biology Stem Cells Cell Differentiation
Acesso em linha:	http://www.sciencedirect.com/science/article/pii/S2666166721001581
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