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Isolation of Murine Alveolar Type II Epithelial Cells

We have optimized a protocol for isolation of alveolar type II epithelial cells from mouse lung. Lung cell suspensions are prepared by intratracheal instillation of dispase and agarose followed by mechanical disaggregation of the lungs. Alveolar type II epithelial cells are purified from these lung...

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Autori principali: Fan Sun, Gutian Xiao, Zhaoxia Qu
Natura: Artigo
Lingua:Inglês
Pubblicazione: Bio-protocol LLC 2017-05-01
Serie:Bio-Protocol
Accesso online:https://bio-protocol.org/en/bpdetail?id=2288&type=0
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