Generation of a heterozygous knockout human embryonic stem cell line for the OCIAD1 locus using CRISPR/CAS9 mediated targeting: BJNhem20-OCIAD1-CRISPR-39

Ovarian carcinoma immuno-reactive antigen domain containing 1 (OCIAD1) single copy was knocked out generating an OCIAD1 heterozygous knockout human embryonic stem line named BJNhem20-OCIAD1-CRISPR-39. The line was generated using CRISPR-Cas9D10A double nickase knockout strategy (Mali et al., 2013).

Bibliografische gegevens

Hoofdauteurs:	Deeti K. Shetty, Maneesha S. Inamdar
Formaat:	Artigo
Taal:	Inglês
Gepubliceerd in:	Elsevier 2016-03-01
Reeks:	Stem Cell Research
Online toegang:	http://www.sciencedirect.com/science/article/pii/S1873506115002172
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