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Cloning of lactoferrin gene of mice and the difference of lactoferrin gene expression in mammary gland at different lactation stages

Total RNA was extracted from mammary gland of mice. Two primers were designed and synthesized according to the murine lactoferrin gene reported in Genbank. A cDNA fragment about 897 bp in length was obtained through RT-PCR. The PCR product was cloned into pGEM-T vector and then sequenced. Sequence a...

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Autori principali: WANG Yi-zhen, HAN Fei-fei, HUANG Hai-qing
Natura: Artigo
Lingua:Inglês
Pubblicazione: Zhejiang University Press 2004-01-01
Serie:浙江大学学报. 农业与生命科学版
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Accesso online:https://www.academax.com/doi/10.3785/1008-9209.2004.01.0083
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