CRISPR/Cas9-Directed Reassignment of the GATA1 Initiation Codon in K562 Cells to Recapitulate AML in Down Syndrome

Using a CRISPR/Cas9 system, we have reengineered a translational start site in the GATA1 gene in K562 cells. This mutation accounts largely for the onset of myeloid leukemia in Down syndrome (ML-DS). For this reengineering, we utilized CRISPR/Cas9 to generate mammalian cell lines that express trunca...

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Detalhes bibliográficos

Principais autores:	Kevin M. Bloh, Pawel A. Bialk, Anilkumar Gopalakrishnapillai, E. Anders Kolb, Eric B. Kmiec
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Elsevier 2017-06-01
coleção:	Molecular Therapy: Nucleic Acids
Assuntos:	gene editing Gata1s protein acute myeloid leukemia in down syndrome CRISPR/Cas9
Acesso em linha:	http://www.sciencedirect.com/science/article/pii/S2162253117301592
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