A Simple, Rapid, and Effective Heparinase Protocol to Enable Nucleic Acid Study from Frozen Heparinized Plasma

Cell-free RNAs (cfRNAs) are promising analytes as non-invasive biomarkers and have even greater potential if tied in with metabolomics. Plasma is an optimal source for cfRNAs but is often derived from a variety of anticoagulants. Plasma obtained in heparin is suitable for metabolomics but is difficu...

Ausführliche Beschreibung

Bibliografische Detailangaben

Hauptverfasser:	Rownock Afruza, Nicole Minerva, Justin B. Lack, Moumita Chakraborty, James A. Haddad, Rabab O. Ali, Christopher Koh, Elliot B. Levy, Ohad Etzion, Theo Heller
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	MDPI AG 2023-11-01
Schriftenreihe:	Methods and Protocols
Schlagworte:	cfRNAs cell-free RNAs qPCR quantitative polymerase chain reaction RT-qPCR reverse transcription–quantitative polymerase chain reaction
Online-Zugang:	https://www.mdpi.com/2409-9279/6/6/112
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