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A robust method for autofluorescence-free immunofluorescence using high-speed fluorescence lifetime imaging microscopy

Abstract Autofluorescence from endogenous biomolecules presents a significant challenge in immunofluorescence microscopy, often severely hindering the detection of specific fluorescence signals. While various techniques, including chemical- and quencher-based methods, photobleaching, and digital-bas...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Wonsang Hwang, Tyler McPartland, Sinyoung Jeong, Conor L. Evans
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Nature Portfolio 2025-02-01
Cyfres:Scientific Reports
Pynciau:
Mynediad Ar-lein:https://doi.org/10.1038/s41598-025-89142-6
Tagiau: Ychwanegu Tag
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