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The LAMP-CRISPR-Cas13a technique for detecting the CBASS mechanism of phage resistance in bacteria

IntroductionAntimicrobial resistance (AMR) is a major public health threat, driving the need for alternative treatments such as phage therapy. However, bacterial defense mechanisms, often regulated by the quorum sensing (QS) network and encoded in genomic islands (GIs), can generate phage-resistant...

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Principais autores: Concha Ortiz-Cartagena, Patricia Fernández-Grela, Lucia Armán, Lucía Blasco, Daniel Pablo-Marcos, Inés Bleriot, Laura Fernández-García, Clara Ibarguren-Quiles, Felipe Fernández-Cuenca, Antonio Barrio-Pujante, Belén Aracil, Jorge Calvo-Montes, María Tomás
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2025-03-01
coleção:Frontiers in Microbiology
Assuntos:
phage resistance
CBASS systems
CRISPR
Cas13a
Purification-free
Acesso em linha:https://www.frontiersin.org/articles/10.3389/fmicb.2025.1550534/full
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