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Development of a gradient method for sulfamethoxazole, trimethoprim, isoniazid, and pyridoxine hydrochloride in rabbit plasma through QbD-driven investigation

Abstract The current study developed a method for quantifying four drugs—Sulfamethoxazole, Trimethoprim, Isoniazid, and Pyridoxine—in rabbit plasma. The method uses gradient liquid chromatography based on analytical quality by design. To achieve separation, a Eclip Plus C18 (250 mm × 5 mm, 4.6 µm) c...

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Principais autores: Premsagar K M, Bhagyalakshmi C, Piyong Sola, Akramul Ansary, Tridib Kumar Das, T. Yunus Pasha, Koushik Nandan Dutta, Ramesh B, Manish Majumder
Formato: Artigo
Idioma:Inglês
Publicado: Nature Portfolio 2024-10-01
Series:Scientific Reports
Assuntos:
Bioanalytical quality by design
Gradient elution
Sulfamethoxazole
Trimethoprim
Isoniazid
Pyridoxine
Acceso en liña:https://doi.org/10.1038/s41598-024-77062-w
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