Simultaneous visualization of extrinsic and intrinsic axon collaterals in Golgi-like detail for mouse corticothalamic and corticocortical cells: a double viral infection method

Here we present a novel tracing technique to stain projection neurons in Golgi-like detail by double viral infection. We used retrograde lentiviral vectors and adeno-associated viral vectors (AAV) to drive TET-ON/TET-OFF system in neurons connecting two regions. Using this method, we successfully l...

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Autors principals:	Akiya eWatakabe, Masafumi eTakaji, Shigeki eKato, Kazuto eKobayashi, Hiroaki eMizukami, Keiya eOzawa, Sonoko eOhsawa, Ryosuke eMatsui, Dai eWatanabe, Tetsuo eYamamori
Format:	Artigo
Idioma:	Inglês
Publicat:	Frontiers Media S.A. 2014-09-01
Col·lecció:	Frontiers in Neural Circuits
Matèries:	corticothalamic aav pyramidal tracer retrograde viral vector axon collateral
Accés en línia:	http://journal.frontiersin.org/Journal/10.3389/fncir.2014.00110/full
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