CRISPR-UnLOCK: Multipurpose Cas9-Based Strategies for Conversion of Yeast Libraries and Strains

Saccharomyces cerevisiae continues to serve as a powerful model system for both basic biological research and industrial application. The development of genome-wide collections of individually manipulated strains (libraries) has allowed for high-throughput genetic screens and an emerging global view...

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Autors principals:	Emily Roggenkamp, Rachael M. Giersch, Emily Wedeman, Muriel Eaton, Emily Turnquist, Madison N. Schrock, Linah Alkotami, Thitikan Jirakittisonthon, Samantha E. Schluter-Pascua, Gareth H. Bayne, Cory Wasko, Megan Halloran, Gregory C. Finnigan
Format:	Artigo
Idioma:	Inglês
Publicat:	Frontiers Media S.A. 2017-09-01
Col·lecció:	Frontiers in Microbiology
Matèries:	CRISPR Cas9 budding yeast libraries marker-less integration gene drive
Accés en línia:	http://journal.frontiersin.org/article/10.3389/fmicb.2017.01773/full
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