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Engineering Streptavidin and a Streptavidin-Binding Peptide with Infinite Binding Affinity and Reversible Binding Capability: Purification of a Tagged Recombinant Protein to High Purity via Affinity-Driven Thiol Coupling.

To extend and improve the utility of the streptavidin-binding peptide tag (SBP-tag) in applications ranging from affinity purification to the reversible immobilization of recombinant proteins, a cysteine residue was introduced to the streptavidin mutein SAVSBPM18 and the SBP-tag to generate SAVSBPM3...

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-д хадгалсан:
Номзүйн дэлгэрэнгүй
Үндсэн зохиолчид: Dawson Fogen, Sau-Ching Wu, Kenneth Kai-Sing Ng, Sui-Lam Wong
Формат: Artigo
Хэл сонгох:Inglês
Хэвлэсэн: Public Library of Science (PLoS) 2015-01-01
Цуврал:PLoS ONE
Онлайн хандалт:http://europepmc.org/articles/PMC4583386?pdf=render
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