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RAA-CRISPR/Cas12a-driven two-tube, one-tube and one-tube-LFS for rapid detection of feline parvovirus

A One-tube method biosensor was developed based on Two-tube recombinase-aid amplification (RAA)-CRISPR/Cas12a system due to the risk of aerosol contamination caused by open-tube operations in the Two-tube method. To further enable naked-eye visualization and portable detection of feline parvovirus (...

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Bibliografische Detailangaben
Hauptverfasser: Guangyu Ge, Zhiyi Chen, Siyuan Liu, Zhonglin Tang, Yulong Yin, Qinghua He
Format: Artigo
Sprache:Inglês
Veröffentlicht: Frontiers Media S.A. 2025-12-01
Schriftenreihe:Frontiers in Veterinary Science
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Online-Zugang:https://www.frontiersin.org/articles/10.3389/fvets.2025.1707332/full
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