Unraveling the salt tolerance of Phi29 DNA polymerase using compartmentalized self-replication and microfluidics platform

In Phi29-α–hemolysin (α-HL) nanopore sequencing systems, a strong electrochemical signal is dependent on a high concentration of salt. However, high salt concentrations adversely affect polymerase activity. Sequencing by synthesis (SBS) requires the use of phi29 polymerase without exonuclease activi...

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Bibliografiske detaljer

Principais autores:	Yaping Sun, Danny Hsu Ko, Jie Gao, Kang Fu, Yaping Gao, Qiwen Zhang, Salem Baldi, Tao Hong, Igor Ivanov, Yun He, Hui Tian
Format:	Artigo
Sprog:	Inglês
Udgivet:	Frontiers Media S.A. 2023-11-01
Serier:	Frontiers in Microbiology
Fag:	compartmentalized self-replication (CSR) sequencing by synthesis (SBS) salt tolerance polymerase droplet-based microfluidics conservative amino acids
Online adgang:	https://www.frontiersin.org/articles/10.3389/fmicb.2023.1267196/full
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