Rapid visualization of Clostridioides difficile toxins A and B by multiplex RPA combined with CRISPR-Cas12a

PurposeClostridioides difficile (C. difficile) infection is the most common cause of nosocomial infection, which is a severe challenge in modern medical care. Currently, many laboratory diagnostic methods for C. difficile are available, such as PCR, culture-based tests, and antigen-based tests. Howe...

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Bibliographic Details

Main Authors:	Tong Jiang, Xinyi Hu, Chunhui Lin, Zhaoxin Xia, Wensu Yang, Yi Zhu, Huaming Xu, Hao Tang, Jilu Shen
Format:	Artigo
Language:	Inglês
Published:	Frontiers Media S.A. 2023-03-01
Series:	Frontiers in Microbiology
Subjects:	Clostridioides difficile toxin gene recombinase polymerase amplification CRISPR/Cas12a lateral flow strip point-of-care testing
Online Access:	https://www.frontiersin.org/articles/10.3389/fmicb.2023.1119395/full
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