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Development and validation of a novel triplex droplet digital PCR assay for simultaneous detection of African swine fever virus, pseudorabies virus, and porcine parvovirus

Coinfection with multiple viruses significantly complicates swine disease management. DNA viruses including African swine fever virus (ASFV), pseudorabies virus (PRV), and porcine parvovirus (PPV) are causing reproductive failure in sows, with overlapping clinical presentations that challenge differ...

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Principais autores: Jiarong Yu, Lu Zhu, Yuanyuan Zuo, Shengbin Gao, Qinghua Wang, Jiao Xu, Linlin Fang, Yumeng Liu, Shuang Liu, Xiaozhen Wang, Xiaohua Wang, Yingli Wang, Jingming Li, Jingyue Bao, Zhiliang Wang
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2026-01-01
coleção:Frontiers in Microbiology
Assuntos:
clinical diagnosis
multiplex droplet digital PCR
ASFV
PRV
PPV
Acesso em linha:https://www.frontiersin.org/articles/10.3389/fmicb.2025.1710807/full
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