Detection of an amplification bias associated to Leuconostocaceae family with a universal primer routinely used for monitoring microbial community structures within food products

Abstract Objectives Sequencing of 16S rDNA V3–V4 region is widely applied for food community profiling. However, two different universal forward primers (named here MUYZER-primer1 and KLINDWORTH-primer2) targeting an identical conservative sequence upstream of the V3 region of 16S rRNA gene, and onl...

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Hauptverfasser:	Simon Poirier, Olivier Rué, Gwendoline Coeuret, Marie-Christine Champomier-Vergès, Valentin Loux, Stéphane Chaillou
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	BMC 2018-11-01
Schriftenreihe:	BMC Research Notes
Schlagworte:	V3 universal primer 16S rDNA amplification bias MiSeq pair-end sequencing Food microbiota Leuconostoc genus
Online-Zugang:	http://link.springer.com/article/10.1186/s13104-018-3908-2
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