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A Method for Rapid Polyethyleneimine-Based Purification of Bacteriophage-Expressed Proteins from Diluted Crude Lysates, Exemplified by Thermostable TP-84 Depolymerase

Purification of bacteriophage-expressed proteins poses methodological difficulties associated with the need to process entire culture medium volume upon bacteriophage-induced bacterial cell lysis. We have used novel capsule glycosylase-depolymerase (TP84_26 GD) from bacteriophage TP-84, infecting th...

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Principais autores: Beata Łubkowska, Edyta Czajkowska, Ireneusz Sobolewski, Natalia Krawczun, Agnieszka Żylicz-Stachula, Piotr M. Skowron
Format: Artigo
Sprog:Inglês
Udgivet: MDPI AG 2023-09-01
Serier:Microorganisms
Fag:
biotechnology
depolymerase
capsule
envelope
biofilm
biofilm removal
Online adgang:https://www.mdpi.com/2076-2607/11/9/2340
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