ssDNA recombineering boosts in vivo evolution of nanobodies displayed on bacterial surfaces
Yamal Al-ramahi et al. describe an accelerated laboratory evolution method that applies ssDNA recombineering and bacterial surface display to engineer nanobody epitope recognition. As a proof-of-concept, they evolved TD4 recognition to bind the translocated intimin receptor (TirM) of EPEC pathogens,...
Salvato in:
| Autori principali: | , , , , , , , |
|---|---|
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Nature Portfolio
2021-10-01
|
| Serie: | Communications Biology |
| Accesso online: | https://doi.org/10.1038/s42003-021-02702-0 |
| Tags: |
Nessun Tag, puoi essere il primo ad aggiungerne!!
|