Secretory expression of recombinant human acetylcholinesterase gene in Pichia pastoris and evaluation of its sensitivity to pesticides

Acetylcholinesterase (AChE) is a key enzyme for the detection of organophosphorus and carbamate pesticide residues by enzyme inhibition method. In this study, human acetylcholinesterase (hAChE) was secreted and expressed by Pichia pastoris expression system, and its sensitivity to eight pesticides w...

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Autors principals:	YANG Fangfang, LI Jiahui, ZHANG Sainan, WANG Zhen, LIAO Zhiyin, WANG Shoufeng
Format:	Artigo
Idioma:	Inglês
Publicat:	Zhejiang University Press 2019-06-01
Col·lecció:	浙江大学学报. 农业与生命科学版
Matèries:	human acetylcholinesterase <italic>Pichia pastoris</italic> secretory expression protein purification enzyme activity determination pesticide sensitivity
Accés en línia:	https://www.academax.com/doi/10.3785/j.issn.1008-9209.2018.09.281
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