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ESI mutagenesis: a one-step method for introducing mutations into bacterial artificial chromosomes

A simple and efficient recombineering-based method for introducing point mutations into bacterial artificial chromosomes using an artificial intron cassette. Bacterial artificial chromosome (BAC)–based transgenes have emerged as a powerful tool for controlled and conditional interrogation of protein...

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Hoofdauteurs: Arnaud Rondelet, Andrei Pozniakovsky, Devika Namboodiri, Richard Cardoso da Silva, Divya Singh, Marit Leuschner, Ina Poser, Andrea Ssykor, Julian Berlitz, Nadine Schmidt, Lea Röhder, Gerben Vader, Anthony A Hyman, Alexander W Bird
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Life Science Alliance LLC 2021-02-01
Reeks:Life Science Alliance
Online toegang:https://www.life-science-alliance.org/lookup/doi/10.26508/lsa.202000836
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