Inhibition of PCR Amplification by a Point Mutation Downstream of a Primer
A T→C point mutation is shown to specifically inhibit PCR amplification when compared to wild-type controls in exon H of the factor IX gene. Multiple primers of different lengths and locations were designed to examine this phenomenon. The experiments suggest that poor annealing and/or extension from...
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| Autori principali: | , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Taylor & Francis Group
1997-02-01
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| Serie: | BioTechniques |
| Accesso online: | https://www.future-science.com/doi/10.2144/97222st01 |
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