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Quantification of lipoprotein lipase in mouse plasma with a sandwich enzyme-linked immunosorbent assay

To support in vivo and in vitro studies of intravascular triglyceride metabolism in mice, we created rat monoclonal antibodies (mAbs) against mouse LPL. Two mAbs, mAbs 23A1 and 31A5, were used to develop a sandwich ELISA for mouse LPL. The detection of mouse LPL by the ELISA was linear in concentrat...

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Principais autores: Takao Kimura, Kazuya Miyashita, Isamu Fukamachi, Kumiko Fukamachi, Kazumi Ogura, Erina Yokoyama, Katsuhiko Tsunekawa, Takumi Nagasawa, Michael Ploug, Ye Yang, Wenxin Song, Stephen G. Young, Anne P. Beigneux, Katsuyuki Nakajima, Masami Murakami
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2024-04-01
coleção:Journal of Lipid Research
Assuntos:
lipids
triglycerides
transport
vascular biology
LPL
HTGL
Acesso em linha:http://www.sciencedirect.com/science/article/pii/S0022227524000373
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