Substrate profiling of marine-derived thermotolerant cysteine protease reveals unique cleavage preferences for industrial applications

Abstract Due to their industrial importance, new proteases are constantly being sourced from the marine environment. However, their substrate specificities remain insufficiently studied, restricting the evaluation of their potential applications. Here, we applied multiplex substrate profiling by mas...

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Hlavní autoři:	Victoria Røyseth, Brianna M. Hurysz, Hasan Arsın, Julia M. Vazquez, Anna-Karina Kaczorowska, Anita-Elin Fedøy, Daria Biernacka, Sebastian Dorawa, Tadeusz Kaczorowski, Runar Stokke, Anthony J. O’Donoghue, Ida Helene Steen
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Nature Portfolio 2025-07-01
Edice:	Scientific Reports
Témata:	Marine cysteine protease Globupain Clostripain Multiplex substrate profiling by mass spectrometry Marine bioprospecting Hydrothermal vents
On-line přístup:	https://doi.org/10.1038/s41598-025-11635-1
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