Rapid Sanger sequencing of the 16S rRNA gene for identification of some common pathogens.

Conventional Sanger sequencing remains time-consuming and laborious. In this study, we developed a rapid improved sequencing protocol of 16S rRNA for pathogens identification by using a new combination of SYBR Green I real-time PCR and Sanger sequencing with FTA® cards. To compare the sequencing qua...

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Detalhes bibliográficos

Principais autores:	Linxiang Chen, Ying Cai, Guangbiao Zhou, Xiaojun Shi, Jianhui Su, Guanwu Chen, Kun Lin
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Public Library of Science (PLoS) 2014-01-01
coleção:	PLoS ONE
Acesso em linha:	http://europepmc.org/articles/PMC3925228?pdf=render
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