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Establishment of a genome editing tool using CRISPR-Cas9 ribonucleoprotein complexes in the non-model plant pathogen Sphaerulina musiva

CRISPR-Cas9 is a versatile genome editing system widely used since 2013 to introduce site-specific modifications into the genomes of model and non-model species. This technology is used in various applications, from gene knock-outs, knock-ins, and over-expressions to more precise changes, such as th...

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Principais autores: Joanna Tannous, Cole Sawyer, Md Mahmudul Hassan, Jesse L. Labbe, Carrie Eckert
Format: Artigo
Sprog:Inglês
Udgivet: Frontiers Media S.A. 2023-07-01
Serier:Frontiers in Genome Editing
Fag:
CRISPR-Cas9
RNP
spore-bearing fungi
ascomycetes
trichoderma reesei
Sphaerulina musiva
Online adgang:https://www.frontiersin.org/articles/10.3389/fgeed.2023.1110279/full
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