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In Vitro Inhibition of Influenza Virus Using CRISPR/Cas13a in Chicken Cells

Advances in the field of CRISPR/Cas systems are expanding our ability to modulate cellular genomes and transcriptomes precisely and efficiently. Here, we assessed the Cas13a-mediated targeted disruption of RNA in chicken fibroblast DF1 cells. First, we developed a Tol2 transposon vector carrying the...

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Autors principals: Arjun Challagulla, Karel A. Schat, Timothy J. Doran
Format: Artigo
Idioma:Inglês
Publicat: MDPI AG 2021-06-01
Col·lecció:Methods and Protocols
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Accés en línia:https://www.mdpi.com/2409-9279/4/2/40
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