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Generation of liver metastases in a mouse model using ultrasound-guided intravenous injection

Summary: Here, we present a protocol to generate a murine model of liver metastasis by directly injecting tumor cells into the portal vein under ultrasound guidance. We describe steps for animal and cell preparation and two techniques for injecting tumor cells. One technique is freehand, while the o...

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Principais autores: Amanda Labora, Hailey Lee, Charlotte Chan, Erin Tabornal, Thuc Le, Khalid Rashid, Evan Abt, Takanobu Yamao, Hanna Mandl, Amanda Creech, Alykhan Premji, Luyi Li, Jason Link, Nanping Wu, Caius Radu, Timothy Donahue
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2023-06-01
coleção:STAR Protocols
Assuntos:
Cancer
Cell Biology
Health Sciences
Model Organisms
Acesso em linha:http://www.sciencedirect.com/science/article/pii/S2666166723001211
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