Protocol to isolate nuclei from Chlamydomonas reinhardtii for ATAC sequencing

Summary: The isolation of sufficient amounts of intact nuclei is essential to obtain high-resolution maps of chromatin accessibility via assay for transposase-accessible chromatin using sequencing (ATAC-seq). Here, we present a protocol for tag-free isolation of nuclei from both cell walled and cell...

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Номзүйн дэлгэрэнгүй

Үндсэн зохиолчид:	Indu Santhanagopalan, Antonia Netzl, Tanya Mathur, Alison Smith, Howard Griffiths, Andre Holzer
Формат:	Artigo
Хэл сонгох:	Inglês
Хэвлэсэн:	Elsevier 2024-03-01
Цуврал:	STAR Protocols
Нөхцлүүд:	Genetics Genomics Sequencing Model Organisms Plant sciences Molecular Biology
Онлайн хандалт:	http://www.sciencedirect.com/science/article/pii/S2666166723007311
Шошгууд:	Шошго нэмэх Шошго байхгүй, Энэхүү баримтыг шошголох эхний хүн болох!