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One-step Affinity Purification of the Chloroplast ClpP Complex from the Green Alga Chlamydomonas reinhardtii Using the Strep-tagII Epitope Tag

This protocol describes the affinity purification of the chloroplast ClpP complex from Chlamydomonas reinhardtii. To this purpose, we have created a Chlamydomonas reinhardtii strain in which the chloroplast encoded ClpP1 subunit of the ClpP complex is tagged at its C-terminal end by a strep-tagII pe...

Whakaahuatanga katoa

I tiakina i:
Ngā taipitopito rārangi puna kōrero
Ngā kaituhi matua: Benoit Derrien, Olivier Vallon
Hōputu: Artigo
Reo:Inglês
I whakaputaina: Bio-protocol LLC 2013-01-01
Rangatū:Bio-Protocol
Urunga tuihono:https://bio-protocol.org/en/bpdetail?id=315&type=0
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