Activity-based CRISPR scanning uncovers allostery in DNA methylation maintenance machinery

Allostery enables dynamic control of protein function. A paradigmatic example is the tightly orchestrated process of DNA methylation maintenance. Despite the fundamental importance of allosteric sites, their identification remains highly challenging. Here, we perform CRISPR scanning on the essential...

Celý popis

Podrobná bibliografie

Hlavní autoři:	Kevin Chun-Ho Ngan, Samuel M Hoenig, Hui Si Kwok, Nicholas Z Lue, Pallavi M Gosavi, David A Tanner, Emma M Garcia, Ceejay Lee, Brian B Liau
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	eLife Sciences Publications Ltd 2023-02-01
Edice:	eLife
Témata:	functional genomics crispr–cas9 mutagenesis DNA methylation bioinformatics allostery mammalian cells
On-line přístup:	https://elifesciences.org/articles/80640
Tagy:	Přidat tag Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!