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Application of bioluminescence resonance energy transfer assays in primary mouse neuronal cultures

Summary: Bioluminescence resonance energy transfer (BRET) is widely employed for real-time monitoring of G protein-coupled receptor activity, interactions, and trafficking in heterologous cell lines, yet its use in neuronal systems remains limited. Here, we present a protocol to apply BRET assays to...

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Auteurs principaux: Hanh T.M. Hoang, Kiran George, Mohiuddin Ahmad
Format: Artigo
Langue:Inglês
Publié: Elsevier 2024-09-01
Collection:STAR Protocols
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Accès en ligne:http://www.sciencedirect.com/science/article/pii/S2666166724003939
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