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Rapid Sequencing of Multiple RNA Viruses in Their Native Form

Long-read nanopore sequencing by a MinION device offers the unique possibility to directly sequence native RNA. We combined an enzymatic poly-A tailing reaction with the native RNA sequencing to (i) sequence complex population of single-stranded (ss)RNA viruses in parallel, (ii) detect genome, subge...

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Principais autores: Thidathip Wongsurawat, Piroon Jenjaroenpun, Mariah K. Taylor, Jasper Lee, Aline Lavado Tolardo, Jyothi Parvathareddy, Sangam Kandel, Taylor D. Wadley, Bualan Kaewnapan, Niracha Athipanyasilp, Andrew Skidmore, Donghoon Chung, Chutikarn Chaimayo, Michael Whitt, Wannee Kantakamalakul, Ruengpung Sutthent, Navin Horthongkham, David W. Ussery, Colleen B. Jonsson, Intawat Nookaew
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2019-02-01
coleção:Frontiers in Microbiology
Assuntos:
native RNA
genome
subgenomic mRNA
single-stranded RNA
virus
nanopore sequencing
Acesso em linha:https://www.frontiersin.org/article/10.3389/fmicb.2019.00260/full
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