High-efficiency CRISPR gene editing in C. elegans using Cas9 integrated into the genome.
Gene editing in C. elegans using plasmid-based CRISPR reagents requires microinjection of many animals to produce a single edit. Germline silencing of plasmid-borne Cas9 is a major cause of inefficient editing. Here, we present a set of C. elegans strains that constitutively express Cas9 in the germ...
Guardat en:
| Autors principals: | , , , |
|---|---|
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Public Library of Science (PLoS)
2021-11-01
|
| Col·lecció: | PLoS Genetics |
| Accés en línia: | https://doi.org/10.1371/journal.pgen.1009755 |
| Etiquetes: |
Sense etiquetes, Sigues el primer a etiquetar aquest registre!
|