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A semi-automated pipeline integrating ImageJ/Fiji and StarDist for the reproducible quantification of cellular and optical density metrics in immunofluorescence images of brain tissue

Quantitative immunofluorescence is widely used to assess molecular expression and cellular distribution across biological tissues, yet the analysis of large image datasets remains time-consuming and prone to user-dependent variability. To address these limitations, we herein developed a semi-automat...

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Hlavní autoři: Sandra Isabel Marques, Helena Carmo, Félix Carvalho, João Pedro Silva, Susana Isabel Sá
Médium: Artigo
Jazyk:Inglês
Vydáno: Frontiers Media S.A. 2026-03-01
Edice:Frontiers in Neuroanatomy
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On-line přístup:https://www.frontiersin.org/articles/10.3389/fnana.2026.1778296/full
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