Optimization of Taq DNA polymerase enzyme expression in Escherichia coli
Background: In the present study, we optimized the experimental conditions using pET15b expression vector to obtain large amounts of Taq DNA polymerase. Materials and Methods: Correct framing of the gene in the expression vector pET15b and its orientation were analyzed by digestion and sequencing. P...
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| 主要な著者: | , , , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Wolters Kluwer Medknow Publications
2012-01-01
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| シリーズ: | Advanced Biomedical Research |
| 主題: | |
| オンライン･アクセス: | http://www.advbiores.net/article.asp?issn=2277-9175;year=2012;volume=1;issue=1;spage=82;epage=82;aulast=Moazen |
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