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Optimized Protocol for Microalgae DNA Staining with SYTO9/SYBR Green I, Based on Flow Cytometry and RSM Methodology: Experimental Design, Impacts and Validation

Multiple fluorochromes are extensively used to investigate different microalgal aspects, such as viability and physiology. Some of them can be used to stain nucleic acids (DNA). Well-known examples are SYBR Green I and SYTO 9, the latter of which offers several advantages, especially when combined w...

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Principais autores: Yob Ihadjadene, Thomas Walther, Felix Krujatz
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI AG 2022-09-01
coleção:Methods and Protocols
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Acesso em linha:https://www.mdpi.com/2409-9279/5/5/76
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