Evaluating methods for isolating total RNA and predicting the success of sequencing phylogenetically diverse plant transcriptomes.

Next-generation sequencing plays a central role in the characterization and quantification of transcriptomes. Although numerous metrics are purported to quantify the quality of RNA, there have been no large-scale empirical evaluations of the major determinants of sequencing success. We used a combin...

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Autori principali:	Marc T J Johnson, Eric J Carpenter, Zhijian Tian, Richard Bruskiewich, Jason N Burris, Charlotte T Carrigan, Mark W Chase, Neil D Clarke, Sarah Covshoff, Claude W Depamphilis, Patrick P Edger, Falicia Goh, Sean Graham, Stephan Greiner, Julian M Hibberd, Ingrid Jordon-Thaden, Toni M Kutchan, James Leebens-Mack, Michael Melkonian, Nicholas Miles, Henrietta Myburg, Jordan Patterson, J Chris Pires, Paula Ralph, Megan Rolf, Rowan F Sage, Douglas Soltis, Pamela Soltis, Dennis Stevenson, C Neal Stewart, Barbara Surek, Christina J M Thomsen, Juan Carlos Villarreal, Xiaolei Wu, Yong Zhang, Michael K Deyholos, Gane Ka-Shu Wong
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Public Library of Science (PLoS) 2012-01-01
Serie:	PLoS ONE
Accesso online:	http://europepmc.org/articles/PMC3504007?pdf=render
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