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Routine single particle CryoEM sample and grid characterization by tomography

Single particle cryo-electron microscopy (cryoEM) is often performed under the assumption that particles are not adsorbed to the air-water interfaces and in thin, vitreous ice. In this study, we performed fiducial-less tomography on over 50 different cryoEM grid/sample preparations to determine the...

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Hlavní autoři: Alex J Noble, Venkata P Dandey, Hui Wei, Julia Brasch, Jillian Chase, Priyamvada Acharya, Yong Zi Tan, Zhening Zhang, Laura Y Kim, Giovanna Scapin, Micah Rapp, Edward T Eng, William J Rice, Anchi Cheng, Carl J Negro, Lawrence Shapiro, Peter D Kwong, David Jeruzalmi, Amedee des Georges, Clinton S Potter, Bridget Carragher
Médium: Artigo
Jazyk:Inglês
Vydáno: eLife Sciences Publications Ltd 2018-05-01
Edice:eLife
Témata:
cryoET
single particle
protomo
fiducial-less
air-water
tomography
On-line přístup:https://elifesciences.org/articles/34257
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