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An Optimized Small-Scale Rearing System to Support Embryonic Microinjection Protocols for Western Corn Rootworm, <i>Diabrotica virgifera virgifera</i>

Western corn rootworm (WCR), a major pest of corn, has been reared in laboratories since the 1960s. While established rearing methods are appropriate for maintaining WCR colonies, they are not optimal for performing germline transformation or CRISPR/Cas9-based genome editing. Here we report the deve...

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Main Authors: Fu-Chyun Chu, Pei-Shan Wu, Sofia Pinzi, Nathaniel Grubbs, Allen Carson Cohen, Marcé D. Lorenzen
Format: Artigo
Language:Inglês
Published: MDPI AG 2023-08-01
Series:Insects
Subjects:
insect rearing
rootworm
molecular biology
function genomics
CRISPR
Online Access:https://www.mdpi.com/2075-4450/14/8/683
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