A modular cloning (MoClo) toolkit for reliable intracellular protein targeting in the yeast Saccharomyces cerevisiae

Modular Cloning (MoClo) allows the combinatorial assembly of plasmids from standardized genetic parts without the need of error-prone PCR reactions. It is a very powerful strategy which enables highly flexible expression patterns without the need of repetitive cloning procedures. In this study, we d...

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Autori principali:	Pavel Simakin, Christian Koch, Johannes M. Herrmann
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Shared Science Publishers OG 2023-02-01
Serie:	Microbial Cell
Soggetti:	expression plasmids mitochondrial import modular cloning promoter strength protein targeting split gfp
Accesso online:	http://microbialcell.com/researcharticles/2023a-simakin-microbial-cell/
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