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Mismatched Primer Extension Assays

Steady state kinetic assays have been a reliable way to estimate fidelity of several polymerases (Menendez-Arias, 2009; Rezende and Prasad, 2004; Svarovskaia et al., 2003). The ability to analyze the extension of primers with specific mismatches at the 3ʹ end is a major strength of the mismatched pr...

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Détails bibliographiques
Auteurs principaux: Vasudevan Achuthan, Jeffrey DeStefano
Format: Artigo
Langue:Inglês
Publié: Bio-protocol LLC 2015-06-01
Collection:Bio-Protocol
Accès en ligne:https://bio-protocol.org/en/bpdetail?id=1508&type=0
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