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A versatile enhanced freeze-substitution protocol for volume electron microscopy

Volume electron microscopy, a powerful approach to generate large three-dimensional cell and tissue volumes at electron microscopy resolutions, is rapidly becoming a routine tool for understanding fundamental and applied biological questions. One of the enabling factors for its adoption has been the...

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Autori principali: Sébastien Bélanger, Heather Berensmann, Valentina Baena, Keith Duncan, Blake C. Meyers, Kedar Narayan, Kirk J. Czymmek
Natura: Artigo
Lingua:Inglês
Pubblicazione: Frontiers Media S.A. 2022-08-01
Serie:Frontiers in Cell and Developmental Biology
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Accesso online:https://www.frontiersin.org/articles/10.3389/fcell.2022.933376/full
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