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High-Fidelity and Cost-Effective Engineering of SARS-CoV-2

Efficient reverse genetics systems are essential for understanding SARS-CoV-2 pathogenesis, host–virus interactions, and potential therapeutic interventions. Here, we developed a cost-effective PCR-based reverse genetics platform that splits the SARS-CoV-2 genome into only six bacterial plasmids, en...

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Guardat en:
Autors principals: Marco Olguin-Nava, Thomas Hennig, Charlene Börtlein, Patrick Bohn, Uddhav B. Ambi, Alexander Gabel, Lina M. Günter, Anne-Sophie Gribling-Burrer, Nora Schmidt, Neva Caliskan, Lars Dölken, Mathias Munschauer, Redmond P. Smyth
Format: Artigo
Idioma:Inglês
Publicat: MDPI AG 2025-12-01
Col·lecció:Viruses
Matèries:
SARS-CoV-2
reverse genetic system
spike
NSP9
Accés en línia:https://www.mdpi.com/1999-4915/17/12/1604
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