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A plasmid-transposon hybrid mutagenesis system effective in a broad range of Enterobacteria

Random transposon mutagenesis is a powerful technique used to generate libraries of genetic insertions in many different bacterial strains. Here we develop a system facilitating random transposon mutagenesis in a range of different Gram-negative bacterial strains, including Pectobacterium atroseptic...

Täydet tiedot

Tallennettuna:
Päätekijät: Rita eMonson, Deborah S Smith, Miguel A. Matilla, Kevin eRoberts, Elizabeth eRichardson, Alison eDrew, Neil eWilliamson, Josh eRamsay, Martin eWelch, George P.C. Salmond
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: Frontiers Media S.A. 2015-12-01
Sarja:Frontiers in Microbiology
Aiheet:
Serratia
Plant Pathogen
Enterobacteria
transposon mutagenesis
Dickeya
Gas vesicles
Linkit:http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01442/full
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